whitepaper

Application note: The effect of column hardware on the analysis of synthetic oligonucleotides by LC-MS

Posted: 7 August 2020 | | No comments yet

Therapeutic oligonucleotides represent a recent breakthrough in the pharmaceutical industry.

Improved reliability to synthetic routes coupled with new drug delivery technologies has given a heightened level of interest in these clinically interesting targets. Chromatographically, they present a challenge due to their polar nature and phosphodiester backbone. As such, ion-pair reversed phase chromatography (IP-RPLC) is necessary, typically using mobile phase consisting of an alkylamine ion pair and perfluorinated alcohol such as HFIP.

    To read this application note in full, please complete the form below. By clicking submit you confirm that you accept our terms and conditions and privacy policy.


    *


    *


    *


    *


    *


    *


    *


    *

    This content is provided to you for free thanks to the kind support of our sponsor: Phenomenex