Application note: High-throughput imaging and analysis of spheroids

High-throughput fluorescence imaging and analysis of spheroids – tips, tricks, and tools to efficiently grow, stain, image, and analyze 3D cell cultures

With optimal cell culture reagents, robust fluorescent reagents and assays, and high-performance fluorescence imaging and detection systems, switching from 2D to 3D cell culture can be easily accomplished even in standard laboratory settings. Because 3D spheroids have a cellular environment and other features that more closely resemble tumors and in vivo models, research on these cultures can provide more relevant data and findings that apply to intact biological systems, enhancing research in drug discovery, cancer biology, and other critical areas.