Application note: Production of human induced pluripotent stem cell-derived cortical neurospheres in the DASbox^® Mini Bioreactor System

There is substantial interest in the scientific community in using human induced pluripotent stem cell (hiPSC)-derived neural cells to study basic mechanisms of brain development, neuronal function, and drug-induced effects. In vitro-generated brain organoids are promising models, because they show a remarkable degree of maturation and can be kept in culture for up to 20 months.