Application note: Real-time detection of Gs and Gi Signalling in living cells
Posted: 20 June 2018 | BMG Labtech GmbH | No comments yet
Genetically-encoded biosensors from Montana Molecular allow for detection of GPCR signalling…
HEK293 cells that expressed the biosensor were treated with Isoproterenol, a well-known activator of the G protein subunit Gs. The resulting increase in cAMP was mirrored by changes in fluorescence intensity. Similarly, upon addition of Quinpirole that acts on the inhibitory G-protein subunit Gi, a drop of fluorescence intensity was detected which represented the expected decrease in cAMP.
Detection of Montanta Molecular’s biosensors with the CLARIOstar® microplate reader resulted in high Z’ factors which prove the robustness of the assay.
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