Application note: Cellartis® Power™ Primary HEP Medium – extend functionality of your primary hepatocytes to 4 weeks

Posted: 7 June 2018 | | No comments yet

Human primary hepatocytes (hphep cells) are the gold standard for in vitro evaluation of drug metabolism, drug-drug interactions, safety assessment of drug candidates, and disease modeling.

However, a significant limitation of hphep cells is their rapid loss of function when cultured in vitro. To enable long-term cultures of primary hepatocytes in user-friendly 2D-culture formats, we developed Cellartis Power Primary HEP Medium (Power HEP medium), a new medium that maintains healthy, functional human primary hepatocytes for up to four weeks in conventional 2D cultures – without the need of overlays or sandwich cultures – overcoming a key limitation of hphep cells. Furthermore, the recommended culture schedule is weekend-free, requiring media changes only on Mondays, Wednesdays, and Fridays.

This application note is restricted - login or subscribe free to access

DTR Issue 3 2021 MiniMagThank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.

Why subscribe? Join our growing community of thousands of industry professionals and gain access to:

  • quarterly issues in print and/or digital format
  • case studies, whitepapers, webinars and industry-leading content
  • breaking news and features
  • our extensive online archive of thousands of articles and years of past issues
  • ...And it's all free!

Click here to Subscribe today Login here