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HTS can be carried out in a number of formats, ranging from simple biochemical (target-based) to whole animal screening assay formats. Horst Flotow, from Hit Discovery Constance, discusses the benefits and practicalities of, and recent progress in, whole animal screening. Madhu Lal-Nag, National Center for Advancing Translational Sciences, and Anton…
High throughput screening (HTS) has become an integral and productive part of early-stage drug discovery, delivering hits that were the starting points for hit-to-lead programmes and resulting in a number of marketed drugs. HTS can be carried out in a number of formats, ranging from simple biochemical (target-based) to whole…
Two-dimensional monolayer cell proliferation assays for cancer drug discovery have enabled large-scale screens. But they represent a simplified view of oncogenes or tumour suppressor genes as the genetic drivers of cancer cell proliferation.
21 December 2016 | By DiscoverX Corporations
Optimized Assays for a Multitude of GPCR Applications...
In this Screening In-Depth Focus, scientists from the Assay Development and Screening Platform of Helmholtz Zentrum München provide an overview of different high-throughput screening (HTS)-applicable assay types that can be applied to drug discovery campaigns. Plus an interview with Lee Babiss, CEO of drug discovery firm X-Rx, on the benefits…
The covalent modification of proteins by the attachment of ubiquitin (ubiquitination) is best known for its function to label proteins for proteasomal degradation. However, ubiquitination also plays non-proteasomal roles during signal transduction, DNA repair and protein sorting...
In this application note, Pall Laboratory evaluate the performance of the Pall AcroPrep Advance 96-Well Filter Plates for Nucleic Acid Binding (Pall NAB plate), which contain a silica-based quartz glass fiber media for the isolation of total RNA...
In this application note, Pall Laboratory describe the use of AcroPrep Advance 96-well Long Tip Filter Plate for Nucleic Acid Binding...
4 October 2016 | By Cisbio
Rapid advancements have been made in understanding the key role epigenetic modifications play in regulating cell activity and their implications on cell health and disease progression...
29 September 2016 | By Intellicyt
This webinar highlights the use of the iQue Screener for high-throughput screening of stem cells, small molecules and CRISPR functional screening, presented by David Sykes, M.D., Ph.D., Researcher from Massachusetts General Hospital and Thibault Jonckheere, CEO and Séverine Giltaire, Senior Scientist from ImmunXperts...
In vitro high-throughput cellular target-based assays are commonly used for hit identification targeting G-protein coupled receptors (GPCR). Agonist and antagonist identification of GPCRs often relies on the screening of large collections of chemicals using miniaturised in vitro cell-based functional assays. In the panel of assays, calcium mobilisation measured by aequorin…
Starting in the 1970s, the drug discovery research process changed dramatically. Our understanding of biological targets and pathways grew and the largely empirical and target agnostic in vivo pharmacology approach was replaced with target-centric methods...
22 June 2016 | By Victoria White, Digital Content Producer
TB Alliance and Takeda are to explore hits generated from a high-throughput screening programme conducted to find novel compounds to treat tuberculosis...
As a stem cell divides, two daughter cells are produced that either possess stem cell characteristics or become a cell with a more specialised function. Cellular markers have been identified that can be used to describe the phenotype of all stem cell progeny. However, the development of tools that allow…
