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Application note: A novel way to expedite antibody discovery

Posted: 15 June 2018 | | No comments yet

Analyse isotype titer and cell health in one assay…

In the antibody discovery workflow, primary screens identify IgG expressing mouse hybridoma and/or B cell clones with specific antigen binding attributes which are subsequently characterised through IgG isotyping, antibody quantification, and cell health assays. This multi-step process yields the critical information needed to identify and isolate the correct genetic material for downstream production clone creation.

The Intellicyt Mouse IgG Type and Titer Kit expedites this traditional workflow through a single, multiplexed assay that simultaneously quantifies IgG isotype, IgG quantity per isotype, total IgG secretion, cell count and cell health.