Recommended

Find out more on ‘AI & Informatics: Drug discovery and development’ in our exclusive report
Download your complimentary copy of our latest digital journal
Free membership: sign up today to access all of our exclusive content
Hear from industry leaders in our upcoming webinars – reserve your place today!
whitepaper

Case Study: Characterisation of hiPSCs by Phenotypic Screening

Posted: 8 March 2018 | | No comments yet

Case study detailing a simple, highly reproducible phenotypic screen to characterise human induced pluripotent stem cells…

Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics.

The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterisation and data analysis tools.

This case study details a phenotypic screen developed by HipSci to characterise human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact

It describes how:

  • high-content analysis enables image-based phenotyping of human induced pluripotent stem cells
  • to distinguish single cells from cells in clumps or colonies
  • secondary data analysis tools allow a better understanding of multiparametric datasets such as phenotypic profiles.

The rest of this case study is restricted - login or subscribe free to access

DTR Issue 3 2021 MiniMagThank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.

Why subscribe? Join our growing community of thousands of industry professionals and gain access to:

  • quarterly issues in print and/or digital format
  • case studies, whitepapers, webinars and industry-leading content
  • breaking news and features
  • our extensive online archive of thousands of articles and years of past issues
  • ...And it's all free!

Click here to Subscribe today Login here