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An assay is an analytical procedure in laboratory medicine, pharmacology and molecular biology for measuring the activity of a target entity.
Three-dimensional cell cultures (spheroids, organoids) are becoming widely used as a new predictive tool in early drug discovery. The use of 3D cell cultures is believed to provide a more physiologically relevant response than monolayer (2D) cell cultures because they closely mimic the extracellular matrix and cell-cell interactions that occur…
An immunoassay developed to analyse small molecules has shown 50 times greater accuracy than current systems of analytical techniques...
The basic premise of drug discovery screening necessitates that the biological assays upon which it depends can be performed in a reproducible manner. In addition, the techniques employed must generate results that are biologically relevant and actionable.
The Small Molecule Screening Facility (SMSF) at the University of Wisconsin Madison is a comprehensive high-throughput screening (HTS) centre where researchers have investigated antibiotic-resistant bacterial infections and screening for protein-protein interactions using HTS methods.
For drug discovery and biomarker research, screening campaigns are employed to identify potential new treatments for diseases and to answer questions that remain unknown in the scientific community.
Contract Research Organisations rely on microplate readers for effective hit discovery. Ian Waddell, of Charles River Laboratories, explains what his needs are.
Many drugs fail in clinical trials due to toxicity-related issues; therefore, drug-mediated cellular toxicity should be determined at an early stage in the drug discovery value chain. There are many assay systems that can be utilised to measure cellular toxicity. In this article we demonstrate that intracellular ATP concentrations are…
There is substantial interest in the scientific community in using human induced pluripotent stem cell (hiPSC)-derived neural cells to study basic mechanisms of brain development, neuronal function, and drug-induced effects. In vitro-generated brain organoids are promising models, because they show a remarkable degree of maturation and can be kept in…
This technical note demonstrates the feasibility of using the Thermo Scientific™Fluoroskan™ or Fluoroskan FL instruments with the Invitrogen™ Quant-iT™ dsDNAhigh-sensitivity assays. Sensitive and specific quantification of double-stranded DNA is carried out in microplate format. This is part of a series of technical notes aiming to help customers choose the best…
With breakthroughs in molecular engineering and antibody humanisation, monoclonal antibodies (mAb) are one of the fastest-growing classes of biopharmaceuticals for multiple clinical indications including cancer, cardiovascular disease, autoimmune disorders and infectious disease. Most therapeutic antibody candidates are initially generated using hybridoma technology or primary B-cell screening after antigen immunisation.
Drug target identification has a key role in the drug discovery value chain. A critical step in the development of pharmaceuticals is identifying the direct targets of potential drug candidates as well as distinguishing any secondary or off-target effects...
Reporter gene assays have greatly helped our understanding of gene regulation and signal transduction in vitro and in vivo.
Laura Kilpatrick, Chloe Peach and Carl White from the University of Nottingham talk about cancer research and how they use our microplate readers PHERAstar and CLARIOstar.
In this issue: Omics-informed drug target discovery in combating emerging infectious diseases, measuring intracellular ATP levels to access compound-mediated cellular toxicity, and turning organoids into physiologically relevant high-content assays for drug discovery.
YEATS domains are epigenetic regulators and are recognised as readers of histone post-translational modifications (HPTM) alongside bromodomains, PHD fingers, and others.
