Application note: Using SYPRO® Orange to Detect Protein Melting Temps 1SHARES Share via Pinterest Reddit Buffer Xing WhatsApp Flipboard Posted: 18 November 2019 | Analytik Jena | No comments yet Investigation of thermal protein stability in high throughput using the application of thermal shift assay using SYPRO ® Orange in 96 well format. The stability of proteins depends on ligand interactions, buffer conditions or changes in conformation, which is traditionally investigated by time-consuming Circular Dichroism (CD) Spectroscopy. In addition, the thermal shift assay is based on temperature-induced denaturation and can be monitored using SYPRO® Orange. This fluorescence dye is a naturally quenched dye and interacts with the hydrophobic core of proteins which is exposed after denaturation. Through this interaction, the SYPRO® Orange dye releases a fluorescence signal. The performance of a melting curve by using the qTOWER3 real-time PCR thermal cycler thus allows an easy and fast determination of protein melting temperatures. To read this Application note in full, please complete the form below. By clicking submit you confirm that you accept our terms and conditions and privacy policy. First Name * Surname * Job Title * Organisation * Town/City * Country * Email * Telephone * * This content is provided to you for free thanks to the kind support of our sponsor: Analytik Jena I am happy for my details to be shared with the sponsor(s) of this content *—Please choose an option—YesNo Related content from this organisation Sample preparation automation in label-free compound profiling Scalable solutions for automated sample preparation in label-free compound profiling Application note: Automated NGS library preparation on CyBio FeliX Application note: qPCR to Measure Minute Volumes in High Throughput Application note: Using SYPRO® Orange to Detect Protein Melting Temps Related topicsHit-to-Lead, Screening, Targets Related organisationsAnalytik Jena
