Application note: Orthogonal approaches for studying bacterial biofilms using photometric detection

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Posted: 19 October 2018 | Thermo Fisher Scientfic | No comments yet

Application note: Orthogonal approaches for studying bacterial biofi lms using photometric detection with a Multiskan Sky Microplate Spectrophotometer and brightfi eld imaging with an EVOS FL Auto Imaging System

This application note aims to demonstrate the benefits of using orthogonal approaches for studying Staphylococcus aureus biofilms—in particular, their susceptibility to antimicrobial agents…

This is exemplified by performing a single adherence assay based on crystal violet staining, which allows both quantification of biofilm biomass and imaging of the attached cells. In the workflow presented here, the measurement of the photometric signal from the biomass-bound crystal violet dye was performed with a Thermo Scientific™ Multiskan™ Sky Microplate Spectrophotometer, which was complemented with the brightfield imaging of the microbial attachment pattern using an Invitrogen™ EVOS™ FL Auto Imaging System.

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Application note: Orthogonal approaches for studying bacterial biofilms using photometric detection

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Application note: Orthogonal approaches for studying bacterial biofilms using photometric detection

Please complete the form below to download this free App Note:

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