Application note: Simultaneous dual phosphoprotein target analysis with Alpha Terbium SureFire Ultra Multiplex

This application note discusses how the Alpha SureFire® Ultra™ Multiplex technology provides a phosphoprotein multiplexing detection platform that is truly homogeneous and high throughput.

Evaluation of pharmaceutical compound efficacy and safety in regulating cell behaviour can involve the study of multiple signal transduction pathways. Protein phosphorylation is a very useful readout of cellular activation or inhibition, and these pathways are commonly targeted for therapeutic modulation of disease. Measurement of more than one cellular target can provide greater confidence of positive compound hits on the cellular target of interest, through examination of an unrelated pathway or an upstream target that should be unaffected by the drug.

Alpha SureFire® Ultra™ Multiplex technology provides a phosphoprotein multiplexing detection platform that is truly homogeneous and high throughput. Terbium SureFire Ultra kits are formulated to be combined with AlphaLISA® SureFire Ultra kits to allow the measurement of two selected phosphoprotein targets.