List view / Grid view

Assays

 

An assay is an analytical procedure in laboratory medicine, pharmacology and molecular biology for measuring the activity of a target entity.

article

Transcriptional reprogramming rescues vascular fate of misidentified pluripotent cell‑derived epithelial cells

22 June 2021 | By

A team of scientists has found that a type of cell derived from human stem cells and widely used for brain research and drug development may have been leading researchers astray for years. Here, Dr Raphaël Lis from Weill Cornell Medicine explains how forcing the activity of three known endothelial cell transcription…

article

Covalent fragment screening in cell-based phenotypic models of disease: a collaborative approach

22 June 2021 | By , , , ,

The application of chemical perturbation approaches in phenotypic models is often used to identify protein targets for therapeutic discovery. Increasingly, small molecule fragments which covalently bind to their protein targets are being used to explore the druggable proteome and the resulting fragment‑protein interactions are characterised by chemoproteomic techniques. In this…

article

Why some screening hits are a PAIN

22 June 2021 | By

A major challenge during high-throughput and fragment screening is the potential for identifying ‘frequent hitters’ – compounds that affect unrelated targets. Matthew Lloyd from the University of Bath explains why these hits can arise during drug discovery and how machine learning could be the answer to identifying these compounds.