USC Stem Cell researchers identify genes that affect blood stem cell development

Even though the transplantation of blood stem cells, also known as bone marrow, has saved many lives over many decades, the genes that control the number or function of blood stem cells are not fully understood.

However, in a new study, the University of Southern California (USC) Stem Cell labs of Hooman Allayee and Gregor Adams uncovered genes that affect blood stem cell development and maintenance.

The Allayee and Adams labs performed a genetic screen of a collection of more than 100 mouse strains that are commonly used in laboratories, called the hybrid mouse diversity panel.

Mice lacking the Hopx gene are ineffective blood stem cell donours

The researchers found that different strains have different numbers of several important sub-populations of blood stem cells, including those called “short-term HSCs,” which are responsible for the formation of red and white blood cells in adults. The activation of a gene called Hopx is associated with an increased number of short-term HSCs. The researchers further proved this finding by showing that mice lacking the Hopx gene form fewer short-term HSCs and are ineffective bone marrow donors.

“Short-term HSCs are the major stem cells in the adult bone marrow, so finding new genetic regulators of this subpopulation may have clinical benefits,” said Adams.

More broadly, the researchers have shown that the hybrid mouse diversity panel can be used to find genes that would otherwise go unnoticed.

“This powerful genetics platform has the potential to reveal the genes underlying other stem cell populations or a wide range of diseases that would be difficult to study in humans,” said Allayee.

The study findings are published in Stem Cell Reports.